{"id":4517,"date":"2021-05-25T07:40:36","date_gmt":"2021-05-25T07:40:36","guid":{"rendered":"https:\/\/didsabz-co.com\/?p=4517"},"modified":"2021-05-25T07:42:52","modified_gmt":"2021-05-25T07:42:52","slug":"tissue-staining-2","status":"publish","type":"post","link":"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/","title":{"rendered":"Tissue staining"},"content":{"rendered":"

Tissue staining stage :<\/strong><\/h4>\n

this step includes 2 types of general and specific methods . the general methods is : Hematoxylin-eosin method , with this method the nucleus turns to purple and cytoplasm turns to pink.<\/p>\n

The specific method is that each cell component takes a specific color, which includes staining of frozen sections , connective tissue , neural system , staining for some cytoplasmic materials , staining for enzymes , staining for micro organisms and cytological staining.<\/p>\n

Solutions used in routine staining include : Ethanol, xylene, Hematoxylin , Eosin , Hydrochloric Acid, lithium Carbonate, Distilled water, Acetic Acid.<\/p>\n

Note:<\/h5>\n

0.5 cc of Acetic Acid should be added to every 100 cc of Hematoxyline before use.<\/p>\n

Note:<\/h5>\n

Place the sections in the oven at temperature of 120\u25e6 C for 15-20 minutes before staining , letting them stick completely to the slides.<\/p>\n

Procedure:<\/h5>\n
    \n
  1. pure xylene (1-2 minutes)<\/li>\n
  2. pure xylene (1-2 minutes)<\/li>\n
  3. pure xylene (1-2 minutes)<\/li>\n
  4. \u00a0ethanol 96% (2-3 minutes)<\/li>\n
  5. \u00a0ethanol 80% (2-3 minutes)<\/li>\n
  6. \u00a0ethanol 70% (5 minutes)<\/li>\n<\/ol>\n

    Rinsed with distilled water( temperature about 36 degree)<\/p>\n

    7.\u00a0 Hematoxylins : 5 to 10 minutes, red<\/p>\n

    Rinsed with distilled water<\/p>\n

    8. 1% alcohol acid (prepare 70% alcohol and pour 1 cc of concentrated hydrochloric acid for every 100 cc of alcohol)<\/p>\n

    9. 1% saturated lithium carbonate: several dips (per 100cc of distilled water, one gram of lithium carbonate powder, blue)<\/p>\n

    10. Eosin (4 to 6 minutes)<\/p>\n

    Rinsed with distilled water<\/p>\n

    11. 70% alcohol (1-2 minutes)120<\/p>\n

    12. alcohol 80% (1-2 minutes)<\/p>\n

    13. alcohol 96% (1-2 minutes)<\/p>\n

    14. alcohol 96% (1-2 minutes)<\/p>\n

    15. mixture of alcohol and xylene (1 volume alcohol 96%, 3 volume xylene)<\/p>\n

    16. pure xylene (1-2 minutes)<\/p>\n

    17.pure xylene (1-2 minutes)<\/p>\n

    18.pure xylene (1-2 minutes)<\/p>\n

    \"\u0631\u0646\u06af<\/a><\/p>\n

    Figure1: an example of Kidney Tissue stained by H&E method<\/p>\n

    Note:<\/strong><\/p>\n

    This method of staining strongly highlight the nucleus of the cells that have turned to blue color, & also their morphology & location.<\/p>\n

    \"staining\"<\/p>\n

    Figure2: detection of nucleus , location & morphology of cells by H&E method<\/p>\n

    It is also possible to observe the density of the cells in a specific area & the divided cells by H&E staining under a microscope.<\/p>\n

    \"\u0631\u0646\u06af<\/p>\n

    Figure 3: identification of divided cells & cells density by H&E method.<\/p>\n

    Using this method it is possible to size the cells\u00a0 & observe their structure and that is because all tissues contain blood vessels & this leads to the possibility of comparing the size of the tissue cells.<\/p>\n

    \"tissue
    tissue staining<\/figcaption><\/figure>\n

    Figure 4: estimating the size of the cells by H&E staining method<\/p>\n","protected":false},"excerpt":{"rendered":"

    Tissue staining stage : this step includes 2 types of general and specific methods . the general methods is : Hematoxylin-eosin method , with this method the nucleus turns to purple and cytoplasm turns to pink. The specific method is that each cell component takes a specific color, which includes staining of frozen sections ,[…]\n","protected":false},"author":3,"featured_media":5807,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[109],"tags":[],"yoast_head":"\nTissue staining - \u062f\u06cc\u062f \u0633\u0628\u0632 Tissue staining \u062f\u06cc\u062f \u0633\u0628\u0632<\/title>\n<meta name=\"description\" content=\"Tissue staining stage :this step includes 2 types of general and specific methods . the general methods is : Hematoxylin-eosin method ,\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Tissue staining - \u062f\u06cc\u062f \u0633\u0628\u0632 Tissue staining \u062f\u06cc\u062f \u0633\u0628\u0632\" \/>\n<meta property=\"og:description\" content=\"Tissue staining stage :this step includes 2 types of general and specific methods . the general methods is : Hematoxylin-eosin method ,\" \/>\n<meta property=\"og:url\" content=\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/\" \/>\n<meta property=\"og:site_name\" content=\"\u062f\u06cc\u062f \u0633\u0628\u0632\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-25T07:40:36+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-05-25T07:42:52+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/didsabz-co.com\/wp-content\/uploads\/2021\/05\/OIF.WgVNBy1YMJXiY5GF4u9tWg-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"didsabz_admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"didsabz_admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/\"},\"author\":{\"name\":\"didsabz_admin\",\"@id\":\"https:\/\/didsabz-co.com\/#\/schema\/person\/99946877f44efe4d8579d5e9a0844c78\"},\"headline\":\"Tissue staining\",\"datePublished\":\"2021-05-25T07:40:36+00:00\",\"dateModified\":\"2021-05-25T07:42:52+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/\"},\"wordCount\":384,\"commentCount\":1,\"publisher\":{\"@id\":\"https:\/\/didsabz-co.com\/#organization\"},\"articleSection\":[\"Education\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/\",\"url\":\"https:\/\/didsabz-co.com\/en\/tissue-staining-2\/\",\"name\":\"Tissue staining - 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