{"id":4480,"date":"2021-05-25T05:35:18","date_gmt":"2021-05-25T05:35:18","guid":{"rendered":"https:\/\/didsabz-co.com\/?p=4480"},"modified":"2021-06-19T10:46:55","modified_gmt":"2021-06-19T10:46:55","slug":"he-tissue-staining","status":"publish","type":"post","link":"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/","title":{"rendered":"H&E tissue staining"},"content":{"rendered":"

H&E tissue staining <\/strong><\/p>\n

general H&E staining is a method\u00a0 that is commonly used in most pathology laboratories. It also known as\u00a0 Hematoxylin-eosin.<\/a> This name is actually derived from the name of the material used to perform this method. In this method ,the nucleus and cytoplasm are distinguished and shown with two different colors.<\/p>\n

The cell nucleus turns to purple and the cytoplasm turns to pink.<\/p>\n

In the following, you can see the staining process recommended by Did sabz company<\/a> for pathology slides as follow<\/p>\n

The recommended staining program is as follows:<\/p>\n

Stage1 : pure xylene (2 minute)<\/p>\n

Stage2 : pure xylene(2 minute)<\/p>\n

Stage 3 : ethanol (3 minute)<\/p>\n

Stage 4: ethanol (3 minute)<\/p>\n

Stage 5 :ethanol (3 minute)<\/p>\n

Stage 6: ethanol ( 3 minute)<\/p>\n

Stage 7: distilled water<\/p>\n

Stage 8 : hematoxylin dye ( 10 minute)<\/p>\n

Stage 9: distilled water<\/p>\n

Stage 10: agitation in 1% alcohol acid ( 2 times)<\/p>\n

Stage 11: agitation in 1% lithium carbonate (2 times)<\/p>\n

Stage 12: eosin dye ( 2-5 minute)<\/p>\n

Stage 13: distilled water<\/p>\n

Stage 14: agitation in 70% ethanol (10 seconds)<\/p>\n

Stage 15: agitation in 75% ethanol (10 seconds)<\/p>\n

Stage 16: agitation in 96% ethanol (10 seconds)<\/p>\n

Stage 17:Mix alcohol and xylene (2 to 3 minutes)<\/p>\n

Stage\u00a0 18: Pure Xylene (2 minutes)<\/p>\n

Stage\u00a0 19: Pure Xylene (2 minutes)<\/p>\n

Stage\u00a0 20: Pure Xylene (2 minutes)<\/p>\n

\"tissue<\/a><\/p>\n

Did sabz company’s recommended staining process<\/p>\n","protected":false},"excerpt":{"rendered":"

H&E tissue staining general H&E staining is a method\u00a0 that is commonly used in most pathology laboratories. It also known as\u00a0 Hematoxylin-eosin. This name is actually derived from the name of the material used to perform this method. In this method ,the nucleus and cytoplasm are distinguished and shown with two different colors. The cell[…]\n","protected":false},"author":3,"featured_media":5803,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[109],"tags":[],"yoast_head":"\nH&E tissue staining - \u062f\u06cc\u062f \u0633\u0628\u0632 \u062f\u06cc\u062f \u0633\u0628\u0632 H&E tissue staining<\/title>\n<meta name=\"description\" content=\"H&E tissue staininggeneral H&E staining is a method\u00a0 that is commonly used in most pathology laboratories. It also known as\u00a0 Hematoxylin-eosin.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"H&E tissue staining - \u062f\u06cc\u062f \u0633\u0628\u0632 \u062f\u06cc\u062f \u0633\u0628\u0632 H&E tissue staining\" \/>\n<meta property=\"og:description\" content=\"H&E tissue staininggeneral H&E staining is a method\u00a0 that is commonly used in most pathology laboratories. It also known as\u00a0 Hematoxylin-eosin.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/\" \/>\n<meta property=\"og:site_name\" content=\"\u062f\u06cc\u062f \u0633\u0628\u0632\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-25T05:35:18+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-06-19T10:46:55+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/didsabz-co.com\/wp-content\/uploads\/2021\/04\/1280px-Gastritis_due_to_Helicobacter_pylori_HE_5-1200x800-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"800\" \/>\n\t<meta property=\"og:image:height\" content=\"534\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"didsabz_admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"didsabz_admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/\"},\"author\":{\"name\":\"didsabz_admin\",\"@id\":\"https:\/\/didsabz-co.com\/#\/schema\/person\/99946877f44efe4d8579d5e9a0844c78\"},\"headline\":\"H&E tissue staining\",\"datePublished\":\"2021-05-25T05:35:18+00:00\",\"dateModified\":\"2021-06-19T10:46:55+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/\"},\"wordCount\":189,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/didsabz-co.com\/#organization\"},\"articleSection\":[\"Education\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/\",\"url\":\"https:\/\/didsabz-co.com\/en\/he-tissue-staining\/\",\"name\":\"H&E tissue staining - \u062f\u06cc\u062f \u0633\u0628\u0632 \u062f\u06cc\u062f \u0633\u0628\u0632 H&E tissue staining\",\"isPartOf\":{\"@id\":\"https:\/\/didsabz-co.com\/#website\"},\"datePublished\":\"2021-05-25T05:35:18+00:00\",\"dateModified\":\"2021-06-19T10:46:55+00:00\",\"description\":\"H&E tissue staininggeneral H&E staining is a method\u00a0 that is commonly used in most pathology laboratories. 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