{"id":4308,"date":"2021-05-11T03:39:12","date_gmt":"2021-05-11T03:39:12","guid":{"rendered":"https:\/\/didsabz-co.com\/?p=4308"},"modified":"2021-05-27T04:02:04","modified_gmt":"2021-05-27T04:02:04","slug":"microtome-sectioning-problems","status":"publish","type":"post","link":"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/","title":{"rendered":"Microtome sectioning problems"},"content":{"rendered":"

 <\/p>\n

Microtome sectioning problems<\/strong><\/p>\n

When studying tissue under a Microscope, the thickness of the tissue should be large enough (in microns) to allow light pass through to be studied by Microscope, otherwise no image will be seen in the Microscope. Therefore, to study the tissues, they must be cut with special tool very thin(in microns) so that they can be seen under a Microscope.<\/p>\n

Sample cutting with Paraffin mold is done by a device called Microtome in 5 to 10 microns thickness.<\/p>\n

For this reason, the cutting operation is more sensitive than the previous steps and should be done slowly and carefully.<\/p>\n

The sharpness of Microtome<\/a> blades is directly related to the quality of the sections. In Microscopic studies, there should not be\u00a0 any scratches on length or any non-harmonized shape on the sections.<\/p>\n

Permanent blades should be sharpened periodically with special tools. The edge of the blades should not be visible under a microscope.
\nThe following are some common problems in Microtomy of the tissues and the causes of these problems.<\/p>\n

Some common problems:<\/p>\n

    \n
  1. \n
    Very thin cut<\/h5>\n

    Wrong Microtome settings, applying hot breath to cold blocks to accelerate sectioning , selecting the first section from the .ribbon cut, sectioning at very high speeds, inappropriate preparation, lack of Microtome calibration<\/li>\n<\/ol>\n

     <\/p>\n

    \"\"<\/a>figure 1: very thin section<\/p>\n

    2. Knife lines<\/h5>\n

    \u00a0damaged knife or blade, inappropriate tissue preparation process, presence of hard materials such as calcium in the block, presence of impurities in paraffin, deposition of buffer salts in the sample<\/p>\n

    \"\"<\/a><\/p>\n

    figure 2:existence of knife lines<\/p>\n

      \n
    1. \n
      Rupture<\/h5>\n<\/li>\n<\/ol>\n

      \u00a0Poor preparation process (incomplete dehydration or incomplete clarification and immersion), use of very hot water bath, rough spreading of tissue<\/p>\n

      \"\"<\/a><\/p>\n

      figure 3: tissue rupture<\/p>\n

       <\/p>\n

        \n
      1. \n
        Fine cracks<\/h5>\n<\/li>\n<\/ol>\n

        \u00a0Excessive tissue preparation, very cold block, very fast cut, poor clamping mechanism, improper angle<\/p>\n

        \"\"<\/a><\/p>\n

        figure 4: fine cracks in the section<\/p>\n

          \n
        1. \n
          Twisting<\/h5>\n<\/li>\n<\/ol>\n

          \u00a0Poor float technique, poor fixation, hot block, very thin cut, very hot water bath<\/p>\n

          \"\"<\/a><\/p>\n

          figure 5:existence of torsion in the section<\/p>\n

            \n
          1. \n
            High compression<\/h5>\n<\/li>\n<\/ol>\n

            \u00a0poor preparation process, hot block, very fast cutting, poor paraffin quality, slow cutting edge, very high angle<\/p>\n

            \"\"<\/a><\/p>\n

            figure 6:high compression of the sample<\/p>\n

              \n
            1. \n
              Existence of Bubbles<\/h5>\n<\/li>\n<\/ol>\n

              Existence of Bubbles in Immersion Bath, Poor Immersion Techniques<\/p>\n

              \"\"<\/a><\/p>\n

              figure 7: existence of bubble in the section<\/p>\n

                \n
              1. \n
                Excessive flattening<\/h5>\n<\/li>\n<\/ol>\n

                \u00a0very high bath temperature, prolonged exposure to water, poor fixation (Solution not removed completely)<\/p>\n

                \"\"<\/a><\/p>\n

                figure 8: excessive tissue flattening<\/p>\n

                  \n
                1. \n
                  Contamination<\/h5>\n<\/li>\n<\/ol>\n

                  \u00a0Dirty slides, contaminated immersion bath, labeling pencil effects<\/p>\n

                  \"\"<\/a><\/p>\n

                  figure 9: existence of contamination<\/p>\n","protected":false},"excerpt":{"rendered":"

                    Microtome sectioning problems When studying tissue under a Microscope, the thickness of the tissue should be large enough (in microns) to allow light pass through to be studied by Microscope, otherwise no image will be seen in the Microscope. Therefore, to study the tissues, they must be cut with special tool very thin(in microns)[…]\n","protected":false},"author":3,"featured_media":6367,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[110],"tags":[],"yoast_head":"\nMicrotome sectioning problems - \u062f\u06cc\u062f \u0633\u0628\u0632 \u062f\u06cc\u062f \u0633\u0628\u0632<\/title>\n<meta name=\"description\" content=\"Microtome sectioning problems When studying tissue under a microscope, the thickness of the tissue should be large enough (in microns)\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microtome sectioning problems - \u062f\u06cc\u062f \u0633\u0628\u0632 \u062f\u06cc\u062f \u0633\u0628\u0632\" \/>\n<meta property=\"og:description\" content=\"Microtome sectioning problems When studying tissue under a microscope, the thickness of the tissue should be large enough (in microns)\" \/>\n<meta property=\"og:url\" content=\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/\" \/>\n<meta property=\"og:site_name\" content=\"\u062f\u06cc\u062f \u0633\u0628\u0632\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-11T03:39:12+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-05-27T04:02:04+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/didsabz-co.com\/wp-content\/uploads\/2021\/04\/New-Project-3-e1634533355408.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"800\" \/>\n\t<meta property=\"og:image:height\" content=\"450\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"didsabz_admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"didsabz_admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/\"},\"author\":{\"name\":\"didsabz_admin\",\"@id\":\"https:\/\/didsabz-co.com\/#\/schema\/person\/99946877f44efe4d8579d5e9a0844c78\"},\"headline\":\"Microtome sectioning problems\",\"datePublished\":\"2021-05-11T03:39:12+00:00\",\"dateModified\":\"2021-05-27T04:02:04+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/\"},\"wordCount\":399,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/didsabz-co.com\/#organization\"},\"articleSection\":[\"Guide\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/\",\"url\":\"https:\/\/didsabz-co.com\/en\/microtome-sectioning-problems\/\",\"name\":\"Microtome sectioning problems - 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